Kar-Chun Tan1, Robert D. Trengove2, Garth L. Maker3, Richard P. Oliver4, Peter S. Solomon5,6
1Australian Centre for Necrotrophic Fungal Pathogens, SABC, Faculty of Health Sciences, Murdoch University; 2Separation Science Laboratory, Faculty of Health Sciences, Murdoch University</span><span>Metabolomics Australia, Murdoch University; 3School of Pharmacy, Faculty of Health Sciences, Murdoch University; 4Australian Centre for Necrotrophic Fungal Pathogens, SABC, Faculty of Health Sciences, Murdoch University; 5Metabolomics Australia, Murdoch University; 6Plant Cell Biology, School of Biology (RSBS), The Australian National University
First Published: Metabolomics Journal / The Metabolomics Society, 2009
A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916–1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease.
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